DUAL-KLAS-NIR Measuring System for P700, Plastocyanin, Ferredoxin & Chlorophyll Fluorescence

Reference
DUAL-KLAS-NIR
Brand
Walz

The DUAL-KLAS-NIR follows, online, the redox state of plastocyanin (PC), the photosystem I reaction center (P700) and ferredoxin (Fd), and determines the PC/P700 and Fd/P700 ratios. It is a major step forward from the well-established DUAL-PAM-100.

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DUAL-KLAS-NIR

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Overview

Four NIR wavelength pairs instead of one

The DUAL-PAM-100 relies on a single pair of near-infrared (NIR) wavelengths to follow absorption changes caused by redox changes at the PS I reaction center. The DUAL-KLAS-NIR works with four NIR pairs, which lets it tell apart, without ambiguity, the redox changes of PC, P700 and Fd.

A novel analytical method gives the instrument the in vivo spectral signatures of pure PC, P700 and Fd; that spectral information is what makes online monitoring of their redox changes possible.

A PAM chlorophyll fluorometer as well

Just like the DUAL-PAM-100, the system also measures chlorophyll fluorescence by PAM, with green or blue measuring light. Green light reaches further into the leaf than blue, so green-excited fluorescence carries information from the deeper leaf layers and pairs well with NIR absorption readings, which always cover the whole leaf.

Familiar software

Much of the software is shared with the DUAL-PAM-100, so existing DUAL-PAM-100 users get started with little effort. Automated measuring routines make even complex protocols easy to run.

More information on the manufacturer's site: https://www.walz.com/products/chl_p700/dual-klas-nir/introduction.html

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